9/22/2023 0 Comments M6a dot blot protocol![]() After recognized as the first RNA demethylase, novel functions of FTO have been discovered to control different aspects of biological process, such as dopaminergic signaling and adipogenesis. įTO was originally identified as a regulator for body mass and obesity, as FTO’s deficiency resulted in growth retardation while overactivation of FTO increased food intake and led to obesity. The dysfunction of these m 6A regulators has been implicated to be involved in different physiological and pathological process, particularly in cancer initiation and progression. Briefly, METT元 and METTL4 are “writers” that form a heterodimer to catalyze m 6A methylation together with WTAP YTHDF1/2/3 are identified as “readers” that bind to RNA sequences with m 6A sites and controlled different biological functions, such as RNA decay or mRNA translation, the “erasers” include FTO and another RNA demethylase ALKBH5, which catalyze m 6A demethylation in a Fe(II)- and a-ketoglutarate-dependent manner. Till now, the m 6A networking is well characterized into three subtypes, including “writers”, “readers” and “erasers”. Recently, the fat mass- and obesity-associated protein (FTO) was identified as the first RNA demethylase, expanding the knowledge that the m 6A is a reversible and dynamic process which can regulate not only mRNA stability, splicing, transport, localization and translation, but also microRNA processing and RNA-protein interaction. The N 6-methyladenosine (m 6A) is the most abundant eukaryotic messenger RNA (mRNA) modification which was discovered in the 1970s. Therefore, it is necessary to seek novel biomarkers for early detection and new targets for improved cervical cancer’s therapy and prognosis. Despite the advances in the screening and early prevention of cervical cancer dramatically decreases the overall cervical cancer incidence and mortality in United States and other western countries, a vast majority of patients in low and low-middle income countries are still diagnosed as locally advanced or invasive cervical cancer with high risks of morbidity, metastasis as well as recurrence. Cervical cancer is one of the most prevalent gynecological cancer worldwide, ranking 4th among all female cancers and affects about 450,000 newly diagnosed cases every year.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |